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04.02.2022 | Original Article

Photodynamic therapy mediated by nanoparticles Aluminum Chloro Phthalocyanine in oral squamous carcinoma cells

verfasst von: Lilian Mendes Borburema Cangussu, Ludmilla Regina de Souza, Marcela Gonçalves de Souza, Renato Sobral Monteiro Junior, Luis Alexandre Muehlmann, Paulo Narcizo de Souza, Lucyana Conceição Farias, Sérgio Henrique Sousa Santos, Alfredo Maurício Batista de Paula, André Luiz Sena Guimarães

Erschienen in: Lasers in Medical Science | Ausgabe 5/2022

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Abstract

The aim of this study is to investigate the antineoplastic potential of photodynamic therapy (PDT) mediated by an aluminum-phthalocyanine chloride nanoemulsion (AlPc-NE), against an oral squamous cell carcinoma (OSCC) cell line in vitro. Both OSCC (SCC9) and A431 cell lines were studied in vitro. Four study groups were used: Group 1 (phosphate-buffered saline [PBS]), Group 2 (PBS + 28.3 J/cm² irradiation), Group 3 (AlPc-NE alone), and Group 4 (AlPc-NE + 28.3 J/cm² irradiation). To test the effect of PDT with AlPc-NE, cell viability, migration, and cell death assays were performed. Moreover, the expressions of Ki-67 and TP53 were evaluated using immunoassays. The results showed that PDT mediated by all AlPc-NE concentrations evaluated (i.e., 0.7, 0.35, and 0.17 nM AlPc) significantly reduced the viability of SCC9 cells. Migration and cell death assays also revealed that PDT with AlPc-NE significantly reduced the rate of migration and increased cell death compared to the control groups. In addition, it was found that PDT with AlPc-NE reduced Ki-67 and mutated TP53 immunoexpression. PDT with AlPc-NE is effective in reducing the viability and migration of SCC9. Moreover, PDT with AlPc-NE nanoemulsions reduces the cell proliferation and expression of mutant TP53.

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Titel: Photodynamic therapy mediated by nanoparticles Aluminum Chloro Phthalocyanine in oral squamous carcinoma cells
verfasst von: Lilian Mendes Borburema Cangussu
Ludmilla Regina de Souza
Marcela Gonçalves de Souza
Renato Sobral Monteiro Junior
Luis Alexandre Muehlmann
Paulo Narcizo de Souza
Lucyana Conceição Farias
Sérgio Henrique Sousa Santos
Alfredo Maurício Batista de Paula
André Luiz Sena Guimarães
Publikationsdatum: 04.02.2022
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 5/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03517-z

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Photodynamic therapy mediated by nanoparticles Aluminum Chloro Phthalocyanine in oral squamous carcinoma cells

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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