Introduction
Breakthrough | Sample type (amount) | Segment length | Analyte | Detection (LOD) | Reference(s) |
---|---|---|---|---|---|
Dawn of drug analysis in human hair | Bulk (10 mg) | Whole hair, proximal and distal 2.5-cm segments | Opiates | RIA (1–10 ng) | Baumgartner et al. (1979) [1] |
Single hair analysis | Single hair (0.4 mg) | Whole hair | Phenobarbital | RIA (1.25 ng) | Smith et al. (1981) [2] |
Use of GC–MS | Bulk (5.9–227 mg) | Whole hair | Methamphetamine, amphetamine, antidepressants, nicotine | GC and GC–MS | Ishiyama et al. (1983) [3] |
Bulk (50–100 mg) | Whole hair | Chloroquine, desethylchloroquine | GC–MS | Viala et al. (1983) [4] | |
Sub-ng detection | Single hair | Whole hair | Methamphetamine, amphetamine | GC–MS (10 pg) | Suzuki et al. (1984) [5] |
Use of LC | Bulk (100–200 mg) | Whole hair (4 cm) | Morphine | LC (60 pg) | Marigo et al. (1986) [6] |
Single 1-cm segmental analysis | Single hair | 1-cm Interval | Haloperidol | LC | Matsuno et al. (1990) [12] |
Sub-cm segmental analysis | Bulk (5 strands) and single hair | 2.5-mm Interval | Quinolone derivative | LC | Uematsu et al. (1993) [13] |
Use of LC–MS | Bulk (40 mg) | Whole hair | Buprenorphine | LC–MS (200 pg) | Tracqui et al. (1997) [7] |
Bulk (60 mg) | Whole hair | Methadone | LC–MS | Kintz et al. (1997) [8] | |
Use of MS imaging | Single hair | Laser spot at 30-μm step | Methamphetamine | MALDI-MS imaging | |
Single hair | Laser spot at 1-mm step | Cocaine | MALDI-MS imaging | Porta et al. (2011) [11] | |
Segmentation (1 mm) | Single hair | Interval (1 mm) | Methoxyphenamine | LC–MS/MS (100 fg) | Kamata et al. (2015) [14] |
Sub-mm segmentation | Single hair | Interval (0.4 mm) | Chlorpheniramine | LC–MS/MS (4 fg) | Kuwayama et al. (2016) [15] |
Use of ITM | Single hair | Interval (0.4 mm) | Cold medicines, hay fever medicines | LC–MS/MS (2–40 fg) | Kuwayama et al. (2018) [17] |
Literature search
Analytical methods for drug detection in hair
Usage amount of hair sample
Conventional segmental hair analysis
Micro-segmental hair analysis (MSA)
MSA procedure
Hair collection
Preliminary examination for micro-segmentation of a single hair strand.
Optimized segmentation procedures
Tips for micro-segmentation
Tool/Instrument | Purpose of use | Recommended specification | Commercial product example |
---|---|---|---|
For segmentation | |||
Slicer | To cut a hair strand in round slices | A blade goes down to a stage vertically | Tissue slicer, 51,425 (Stoelting Co., Wood Dale, IL USA) |
Stage | To place a hair strand | As large as possible to place a long hair strand straight Movable using a micrometer scale | Equipped to above slicer |
Micrometer scale | To move a stage at a determined distance | Resolutions: 0.001 mm, 50mm or longer travel distance | A 25-mm micrometer head is equipped to above slicer Micrometer head, 50 mm travel distance, MHH2-50 T (Mitutoyo Corporation, Kawasaki, Japan) |
Razor blade | To cut a hair strand | Stainless steel, single edge, ca. 0.2 mm thick (0.1-mm thick blade is not recommended because it bends easily) | Shaving blade, FHS (FEATHER Safety Razor Co. Ltd., Osaka, Japan) |
Grid paper | To place a hair strand at right angle to a razor blade | Whitish background, light-colored lines ruled into 1-mm squares, A4 size or larger | Section paper, Ho-19 N (KOKUYO Co. Ltd., Osaka, Japan) |
Double-sided adhesive tape | To fix a hair strand | Weakly adhesive power, whitish | NICETACK removable tape NW (NICHIBAN Co. Ltd. Tokyo, Japan) |
Cotton swab | To transfer a hair segment into a tube | Tapered, white color The tip should reach the bottom of a microtube | ASPURE industrial swab, AP-3 (AS ONE Corporation, Osaka, Japan) |
Microtube | To enclose a hair segment | Capacity: 0.1- or 0.2-mL, single tube, flat cap, clear (8-strips, 12-strips, and dome caps are not recommended) | EU 0.1 mL single tube, low profile, BPK77201 (NIPPON Genetics Co. Ltd., Tokyo, Japan) |
Magnifying glass | To see a hair segment carefully | Five-times or more powerful magnification A light is attached | LED stand loupe, SL-23 (AS ONE Corporation) |
Tweezers | To attach a hair strand onto tape straight | Tapered enough to pick up a hair strand | Normal tweezers, thin stainless steel, IPT-02 (AS ONE Corporation) |
White glove | To find a lost hair segment easily when it is on a hand | Thick nylon fiber (nitrile and latex gloves are not recommended because of sweatiness and strong grip performance) | Nylon gloves, EA354AM (ESCO Co. Ltd. Osaka, Japan) |
White paper | To find a lost hair segment easily | Glossy | General white glossy paper, such as the reverse of poster, is available |
Finger cot/glove | To press a hair strand onto tape | Disposable (white gloves described above are not applied to avoid contamination) | General disposable finger cots and gloves are available |
Washing solvent | To wash a razor blade | Volatile, water-soluble solvent such as methanol | General analytical grade solvents are available |
Adhesive swab | To catch a hair segment which is found on a place where it is difficult to collect with cotton swab | Comparatively strong adhesive power, clear or whitish | Peta swab, PS-2520 (ATOM KOUSAN Corporation, Tokyo, Japan) |
Hood | To prevent wind and dust | Large enough to cover the workspace | Portable fume hood, 3–4064-33 (AS ONE Corporation) |
Light stand | To light the workspace | Lighter the better | General products are available |
Lint roller | To clean the workspace before and after segmentation procedure | White adhesive tape | General products are available |
For drug extraction | |||
Tube rack | To array tubes containing a hair segment in turn | Compatible with 0.1-mL and 0.2-mL tubes, 96-well plate sized, with a removable lid It fits a microplate centrifuge rotor and a 12-channel pipette | 96-Well preparation tray, 05–541-55 (Thermo Fisher Scientific Inc., Waltham, MA, USA) |
Dot
label | To label a tube | Diameter at 6.5 mm or less for 0.1- and 0.2-mL tubes | Standard dot label φ6.5 mm white, SDL-25–1 (Shamrock Labels, Bellwood, IL, USA) |
Ultrasonic cleaner | To extract drugs from a hair segment | The chamber holds four or more tube racks The frequency and power of ultrasonic wave, and the chamber temperature are changeable | ASU-10D (AS ONE Corporation) |
Twelve-channel pipette | To add the extraction solution in 12 tubes arranged in a row and transfer extracts into a microplate | Changeable (20–200 μL) | General products are available |
Plate centrifuge | To sink a hair segment to the bottom of a tube | High speed as possible (over 1000 × g) Plates keep horizontal at stopping | PlateSpin (KUBOTA Corporation co., ltd., Tokyo, Japan) |
Plate shaker | To mix extracts and diluents in microplates and 96-well plate-sized tube racks | Compatible with microplates | General products are available |
Ninety-six well plate | To dilute extracts and inject analytical samples into an LC–MS/MS | Standardized by American National Standards Institute (ANSI), V-shaped bottom | PP plate 96 V, MS-3396P (Sumitomo Bakelite Co., Ltd. Tokyo, Japan) |
Plate seal | To seal each well in a 96-well plate | Compatible with a 96-well plate It can be penetrated by the injector of an LC–MS/MS | RAPID EPS (BioChromato, Inc. Fujisawa, Japan) |
Plate cover | To prevent dust into wells and onto a plate seal | Compatible to a 96-well plate | Plate common cover, P38C01N (Stem Corporation, Tokyo, Japan) |
Airtight plastic container | To store an extraction solution and to keep a 12-channel pipette directly | It enables to store solutions for long time Wider than the width of a 12-channel pipette | Unix ware, TLO-10Ag (ASVEL Co. Ltd., Yamatokoriyama, Japan) |