INTRODUCTION
MATERIALS AND METHODS
Chemicals
In Vitro Human Cell Culture
Ex Vivo Culture of Mouse Colonic Explants
Histopathological Analysis of Distal Colonic Explant Tissue
Immunohistochemistry Assessment of Cellular Markers of Apoptosis and Proliferation
RT-PCR of Human Cell Culture and Colonic Explant Tissue
Mouse TLR4 | Forward: 5‘-CTC TGC CTT CAC TAC AGA GAC-3’ Reverse: 5’-TGG ATG ATG TTG GCA GCA ATG-3’ |
Mouse MD2 | Forward: 5‘- GTC CGA TGG TCT TCC TGG CGA GT-3’ Reverse: 5’ GCT TCT CAG ATT CAG TCA ATA TGG G-3’ |
Mouse CD14 | Forward: 5‘- GTC AGG AAC TCT GGC TTT GC-3’ Reverse: 5’ GGC TTT TAC CCA CTG AAC CA-3’ |
Mouse MyD88 | Forward: 5‘- GGA GCC AGA TTC TCT GAT GC-3’ Reverse: 5’ TGT CCC AAA GGA AAC ACA CA-3’ |
Mouse IL-6 | Forward: 5‘- AGT TGC CTT CTT GGG ACT GA-3’ Reverse: 5’ TCC ACG ATT TCC CAG AGA AC-3’ |
Mouse IL-6 Receptor | Forward: 5‘- TGA ATG ATG ACC CCA GGC AC-3’ Reverse: 5’ ACA CCC ATC CGC TCT CTA CT-3’ |
Mouse CXCL2 | Forward: 5‘- AAG TTT GCC TTG ACC CTG AA-3’ Reverse: 5’ AGG CAC ATC AGG TAC GAT CC-3’ |
Mouse CXCR1 | Forward: 5‘- GGG TGA AGC CAC AAC AGA TT-3’ Reverse: 5’ GCA GAC CAG CAT AGT GAG CA-3’ |
Mouse CXCR2 | Forward: 5‘- GCA GAG GAT GGC CTA GTC AG-3’ Reverse: 5’ TCC ACC TAC TCC CAT TCC TG-3’ |
Mouse GAPDH (housekeeper) | Forward: 5‘- CCT CGT CCC GTA GAC AAA ATG-3’ Reverse: 5’ TCT CCA CTT TGC CAC TGC AA-3’ |