nach oben

Erschienen in:

19.11.2021 | Original Article

Impact of photobiomodulation therapy on the morphological aspects of submandibular gland submitted to excretory duct ligation and hypothyroidism: an animal study

verfasst von: Ana Carolina Lemos Pimentel, Tânia Tavares Rodriguez, Manoela Domingues Martins, Luiz Carlos Ramos Filho, Iago Fonseca Silva Mota, Juliana Santos de Carvalho Monteiro, Patricia Rubia Manieri, Antônio Luiz Barbosa Pinheiro, Patrícia Ramos Cury, Jean Nunes Dos Santos

Erschienen in: Lasers in Medical Science | Ausgabe 3/2022

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to evaluate the impact of photobiomodulation therapy (PBMT) on histomorphological aspects of submandibular gland (SMG) submitted to salivary gland duct obstruction in hypothyroid rats. Fifty-six male Wistar rats (250 to 300 g) were divided into 4 groups (n = 14): euthyroid (EU), EU + PBMT, hypothyroid (HYPO), and HYPO + PBMT. Duct obstruction of the left submandibular gland (LSMG) was performed in all animals by a ligature procedure. For the induction of hypothyroidism, total thyroidectomy was performed. PBMT groups received irradiation with AlGaInP diode laser (808 nm, 0.04 W, 0.04cm² spot size, 60 s, 2.4 J per point, 60 J/cm², 1 W/cm²). Irradiation was performed immediately, 24 h, and 48 h after the obstruction of the salivary gland duct, in one point, extra oral and perpendicular to the gland. Animals were sacrificed after 24 h and 72 h after duct ligature. Our results indicated that salivary duct obstruction and hypothyroidism caused negative modifications on the salivary glands’ histomorphology, especially acinar atrophy, after 24 h and 72 h. HYPO + PBMT showed a significant reduction of the inflammatory infiltrate, congested blood vessels, and acinar atrophy in the SMG submandibular salivary gland in 72 h compared to 24 h (p < 0.05). In conclusion, obstruction of the salivary gland excretory duct and hypothyroidism causes severe sialoadenitis with expressive atrophy of the glandular parenchyma. However, PBMT was able to modulate the inflammatory process and delaying acinar atrophy. This study provided insights to better understand the role of the PBMT on the altered salivary gland by duct ligation and associate hypothyroidism.

Hayat NQ, Tahir M, Munir B, Sami W (2010) Effect of methimazole-induced hypothyroidism on histological characteristics of parotid gland of albino rat. J Ayub Med Coll Abbottabad 22:22–27PubMed

Cicatiello AG, Di Girolamo D, Dentice M (2018) Metabolic effects of the intracellular regulation of thyroid hormone: old players, new concepts. Front Endocrinol (Lausanne) 9:474. https://doi.org/10.3389/fendo.2018.00474CrossRef

Patel M, Mishra V, Pawar V et al (2013) Evaluation of acute physiological and molecular alterations in surgically developed hypothyroid Wistar rats. J Pharmacol Pharmacother 4:110–115. https://doi.org/10.4103/0976-500X.110891CrossRefPubMedPubMedCentral

Hayat NQ (2016) The effect of hypothyroidism on the body weight of adult albino wistar rats. J Rawalpindi Med Coll 20:147–149

Chang Y-J, Hwu C-M, Yeh C-C et al (2014) Effects of subacute hypothyroidism on metabolism and growth-related molecules. Molecules 19:11178–11195. https://doi.org/10.3390/molecules190811178CrossRefPubMedPubMedCentral

de Jesus VC, Beanes G, Paraguassú GM et al (2015) Influence of laser photobiomodulation (GaAlAs) on salivary flow rate and histomorphometry of the submandibular glands of hypothyroid rats. Lasers Med Sci 30:1275–1280. https://doi.org/10.1007/s10103-015-1725-6CrossRefPubMed

Jung J-H, Lee C-H, Son SH et al (2017) High prevalence of thyroid disease and role of salivary gland scintigraphy in patients with xerostomia. Nucl Med Mol Imaging 51:169–177. https://doi.org/10.1007/s13139-016-0455-4CrossRefPubMed

Nasr El-Din WA, Abdel Fattah IO (2020) Histopathological and biochemical alterations of the parotid gland induced by experimental hypothyroidism in adult male rats and the possible therapeutic effect of Nigella sativa oil. Tissue Cell 65:101366. https://doi.org/10.1016/j.tice.2020.101366CrossRefPubMed

Naik MM, Vassandacoumara V (2018) Qualitative and quantitative salivary changes and subjective oral dryness among patients with thyroid dysfunction. Indian J Dent Res 29:16–21. https://doi.org/10.4103/ijdr.IJDR_501_16CrossRefPubMed

10.

Rodriguez TT, Dantas VTA, Ramalho MJP (2009) Participation of nitric oxide synthase and cyclooxygenase-2 in the salivary secretion of hypothyroid endotoxemic rats. Revista odonto ciência 24:383–388

11.

Miguel MCC, Andrade ESS, Taga R et al (2002) Hyperplasia of myoepithelial cells expressing calponin during atrophy of the rat parotid gland induced by duct ligation. Histochem J 34:499–506. https://doi.org/10.1023/a:1024761923303CrossRefPubMed

12.

Osailan SM, Proctor GB, McGurk M, Paterson KL (2006) Intraoral duct ligation without inclusion of the parasympathetic nerve supply induces rat submandibular gland atrophy. Int J Exp Pathol 87:41–48. https://doi.org/10.1111/j.0959-9673.2006.00453.xCrossRefPubMedPubMedCentral

13.

Carpenter GH, Osailan SM, Correia P et al (2007) Rat salivary gland ligation causes reversible secretory hypofunction. Acta Physiol (Oxf) 189:241–249. https://doi.org/10.1111/j.1365-201X.2006.01662.xCrossRef

14.

Correia PN, Carpenter GH, Osailan SM et al (2008) Acute salivary gland hypofunction in the duct ligation model in the absence of inflammation. Oral Dis 14:520–528. https://doi.org/10.1111/j.1601-0825.2007.01413.xCrossRefPubMedPubMedCentral

15.

Uzêda-E-Silva VD, Rodriguez TT, Ramalho LMP et al (2017) Does laser phototherapy influence the proliferation of myoepithelial cells in the salivary gland of hypothyroid rats? J Photochem Photobiol B 173:681–685. https://doi.org/10.1016/j.jphotobiol.2017.07.008CrossRefPubMed

16.

De-Castro ICV, de-Jesus AAF, Azevedo RAD et al (2019) Clinicopathological study of sialolithiasis: a retrospective analysis of 11 cases. J Oral Diagn 4:1–6. https://doi.org/10.5935/2525-5711.20190006CrossRef

17.

Takeda Y (1988) Irradiation effect of low-energy laser on rat submandibular salivary gland. J Oral Pathol 17:91–94. https://doi.org/10.1111/j.1600-0714.1988.tb01513.xCrossRefPubMed

18.

Onizawa K, Muramatsu T, Matsuki M et al (2009) Low-level (gallium-aluminum-arsenide) laser irradiation of Par-C10 cells and acinar cells of rat parotid gland. Lasers Med Sci 24:155–161. https://doi.org/10.1007/s10103-008-0541-7CrossRefPubMed

19.

Simões A, Platero MD, Campos L et al (2009) Laser as a therapy for dry mouth symptoms in a patient with Sjögren’s syndrome: a case report. Spec Care Dentist 29:134–137. https://doi.org/10.1111/j.1754-4505.2009.00078.xCrossRefPubMed

20.

Lončar B, Stipetić MM, Baričević M, Risović D (2011) The effect of low-level laser therapy on salivary glands in patients with xerostomia. Photomed Laser Surg 29:171–175. https://doi.org/10.1089/pho.2010.2792CrossRefPubMed

21.

Simões A, de Campos L, de Souza DN et al (2010) Laser phototherapy as topical prophylaxis against radiation-induced xerostomia. Photomed Laser Surg 28:357–363. https://doi.org/10.1089/pho.2009.2486CrossRefPubMed

22.

Vidović Juras D, Lukac J, Cekić-Arambasin A et al (2010) Effects of low-level laser treatment on mouth dryness. Coll Antropol 34:1039–1043PubMed

23.

Saleh J, Figueiredo MAZ, Cherubini K et al (2014) Effect of low-level laser therapy on radiotherapy-induced hyposalivation and xerostomia: a pilot study. Photomed Laser Surg 32:546–552. https://doi.org/10.1089/pho.2014.3741CrossRefPubMed

24.

Terlević Dabić D, Jurišić S, Vučićević Boras V et al (2016) The effectiveness of low-level laser therapy in patients with drug-induced hyposalivation: a pilot study. Photomed Laser Surg 34:389–393. https://doi.org/10.1089/pho.2016.4109CrossRefPubMed

25.

Gonnelli FAS, Palma LF, Giordani AJ et al (2016) Low-level laser therapy for the prevention of low salivary flow rate after radiotherapy and chemotherapy in patients with head and neck cancer. Radiol Bras 49:86–91. https://doi.org/10.1590/0100-3984.2014.0144CrossRefPubMedPubMedCentral

26.

Galiano-Castillo N, Liu L, Lozano-Lozano M et al (2020) Acute and cumulative benefits of Photobiomodulation for xerostomia: a systematic review and meta-analysis. Oral Dis. https://doi.org/10.1111/odi.13648CrossRefPubMed

27.

Anders JJ, Lanzafame RJ, Arany PR (2015) Low-level light/laser therapy versus photobiomodulation therapy. Mary Ann Liebert, Inc., New RochelleCrossRef

28.

Kocherova I, Bryja A, Błochowiak K et al (2021) Photobiomodulation with red and near-infrared light improves viability and modulates expression of mesenchymal and apoptotic-related markers in human gingival fibroblasts. Materials 14:3427. https://doi.org/10.3390/ma14123427CrossRefPubMedPubMedCentral

29.

Simões A, Nicolau J, de Souza DN et al (2008) Effect of defocused infrared diode laser on salivary flow rate and some salivary parameters of rats. Clin Oral Investig 12:25–30. https://doi.org/10.1007/s00784-007-0135-yCrossRefPubMed

30.

Pinheiro ALB (2009) Advances and perspectives on tissue repair and healing. Photomed Laser Surg 27:833–836. https://doi.org/10.1089/pho.2009.2716CrossRefPubMed

31.

Mosca RC, Ong AA, Albasha O et al (2019) Photobiomodulation therapy for wound care: a potent, noninvasive, photoceutical approach. Adv Skin Wound Care 32:157–167. https://doi.org/10.1097/01.ASW.0000553600.97572.d2CrossRefPubMed

32.

Osailan SM, Proctor GB, Carpenter GH et al (2006) Recovery of rat submandibular salivary gland function following removal of obstruction: a sialometrical and sialochemical study. Int J Exp Pathol 87:411–423. https://doi.org/10.1111/j.1365-2613.2006.00500.xCrossRefPubMedPubMedCentral

33.

Scott J, Liu P, Smith PM (1999) Morphological and functional characteristics of acinar atrophy and recovery in the duct-ligated parotid gland of the rat. J Dent Res 78:1711–1719. https://doi.org/10.1177/00220345990780110801CrossRefPubMed

34.

Tamarin A (1979) The leukocytic response in ligated rat submandibular glands. J Oral Pathol 8:293–304. https://doi.org/10.1111/j.1600-0714.1979.tb01831.xCrossRefPubMed

35.

Schindl A, Schindl M, Pernerstorfer-Schön H, Schindl L (2000) Low-intensity laser therapy: a review. J Investig Med 48:312–326PubMed

36.

Hamblin MR (2017) Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS Biophys 4:337–361. https://doi.org/10.3934/biophy.2017.3.337CrossRefPubMedPubMedCentral

37.

Wagner VP, Curra M, Webber LP et al (2016) Photobiomodulation regulates cytokine release and new blood vessel formation during oral wound healing in rats. Lasers Med Sci 31:665–671. https://doi.org/10.1007/s10103-016-1904-0CrossRefPubMed

38.

Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 49:1–17. https://doi.org/10.1016/S1011-1344(98)00219-XCrossRefPubMed

39.

Walski T, Dąbrowska K, Drohomirecka A et al (2019) The effect of red-to-near-infrared (R/NIR) irradiation on inflammatory processes. Int J Radiat Biol 95:1326–1336. https://doi.org/10.1080/09553002.2019.1625464CrossRefPubMed

40.

Fukuoka CY, Simões A, Uchiyama T et al (2017) The effects of low-power laser irradiation on inflammation and apoptosis in submandibular glands of diabetes-induced rats. PLoS ONE 12:e0169443. https://doi.org/10.1371/journal.pone.0169443CrossRefPubMedPubMedCentral

Titel: Impact of photobiomodulation therapy on the morphological aspects of submandibular gland submitted to excretory duct ligation and hypothyroidism: an animal study
verfasst von: Ana Carolina Lemos Pimentel
Tânia Tavares Rodriguez
Manoela Domingues Martins
Luiz Carlos Ramos Filho
Iago Fonseca Silva Mota
Juliana Santos de Carvalho Monteiro
Patricia Rubia Manieri
Antônio Luiz Barbosa Pinheiro
Patrícia Ramos Cury
Jean Nunes Dos Santos
Publikationsdatum: 19.11.2021
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03463-2

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Impact of photobiomodulation therapy on the morphological aspects of submandibular gland submitted to excretory duct ligation and hypothyroidism: an animal study

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2022

Effectiveness of preconditioned adipose-derived mesenchymal stem cells with photobiomodulation for the treatment of diabetic foot ulcers: a systematic review

Laser-assisted hatching zona thinning does not improve the pregnancy outcomes of poor-quality blastocysts in frozen-thawed embryo transfer cycle: a retrospective cohort study

Excimer laser atherectomy combined with drug-coated balloon versus drug-eluting balloon angioplasty for the treatment of infrapopliteal arterial revascularization in ischemic diabetic foot: 24-month outcomes

Correction to: Efficiency and safety of laser‑assisted hatching on vitrified‑warmed blastocyst transfer cycles: a prospective control trial

Low-energy laser therapy application on knee joints as an auxiliary treatment in patients with polyarticular juvenile idiopathic arthritis: a dual-arm randomized clinical trial

Photodynamic therapy for squamous cell carcinoma of the head and neck: narrative review focusing on photosensitizers