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28.04.2023 | Research

Immunoexpression of Autophagy-Related Proteins in Salivary Gland Tumors: An Exploratory Study

verfasst von: Emanuene Galdino Pires, Christany Rodrigues Ferreira, Roberta Barroso Cavalcante, Maria Cássia Ferreira de Aguiar, Ricardo Alves Mesquita, Pollianna Muniz Alves, Cassiano Francisco Weege Nonaka

Erschienen in: Head and Neck Pathology | Ausgabe 3/2023

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Abstract

Background

Autophagy is a cellular survival mechanism involved in several human diseases, but its participation in the development of salivary gland tumors is not fully understood. This study investigated the immunoexpression of autophagy-related proteins (autophagy-related 7 [Atg7], microtubule-associated protein 1 light chain 3A [LC3A], microtubule-associated protein 1 light chain 3B [LC3B], protein p62 [p62], and phosphorylated mammalian target of rapamycin [p-mTOR]) in pleomorphic adenoma (PA), polymorphous adenocarcinoma (PAC), mucoepidermoid carcinoma (MEC), and adenoid cystic carcinoma (ACC) of salivary glands.

Methods

Twenty PAs, 20 PACs, 20 MECs, and 14 ACCs were selected. The percentages of cytoplasmic and nuclear positivity for autophagy-related proteins in neoplastic cells were assessed and correlated with histopathological parameters.

Results

Cytoplasmic immunoexpression of Atg7 was observed in all groups, with high median percentages of positivity. Regarding LC3A and LC3B, cytoplasmic immunoexpression was found in most PACs (95%) and in all cases of PA, MEC and ACC, with the highest percentages of positivity in PACs and PAs (p < 0.005). ACCs exhibited lower cytoplasmic immunoexpression of p-mTOR (p < 0.005) and lower nuclear expression of p62 (p < 0.05) when compared to PAs, PACs and MECs. Low nuclear immunoexpression of Atg7, LC3A and p-mTOR and absence of nuclear staining for LC3B were observed in all groups. Regarding histopathological parameters of PAs, MECs and ACCs, there were no significant differences in the expression of autophagy-related proteins. In all groups, positive correlations were observed between the immunoexpression of some autophagy-related proteins (p < 0.05).

Conclusions

The results suggest the participation of autophagy in the pathogenesis of PA, PAC, MEC, and ACC of salivary glands. Upregulation of autophagy and reduced nuclear translocation of p62 may contribute to the aggressive biological behavior of salivary gland ACC.

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Titel: Immunoexpression of Autophagy-Related Proteins in Salivary Gland Tumors: An Exploratory Study
verfasst von: Emanuene Galdino Pires
Christany Rodrigues Ferreira
Roberta Barroso Cavalcante
Maria Cássia Ferreira de Aguiar
Ricardo Alves Mesquita
Pollianna Muniz Alves
Cassiano Francisco Weege Nonaka
Publikationsdatum: 28.04.2023
Verlag: Springer US
Erschienen in: Head and Neck Pathology / Ausgabe 3/2023
Elektronische ISSN: 1936-0568
DOI: https://doi.org/10.1007/s12105-023-01556-8

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Immunoexpression of Autophagy-Related Proteins in Salivary Gland Tumors: An Exploratory Study

Abstract

Background

Methods

Results

Conclusions

Neu im Fachgebiet Pathologie

Assistierter Suizid durch Infusion von Thiopental

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Background

Methods

Results

Conclusions

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