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Erschienen in: Surgical and Radiologic Anatomy 8/2023

09.06.2023 | Original Article

Guidance in botulinum neurotoxin injection for lower extremity spasticity: Sihler’s staining technique

verfasst von: Hyewon Hu, Min Ho An, Hyung-Jin Lee, Kyu-Ho Yi

Erschienen in: Surgical and Radiologic Anatomy | Ausgabe 8/2023

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Abstract

Spasticity is a motor disease characterized by a velocity-dependent acceleration in muscle tone or tonic stretch reflexes linked to hypertonia. Lower limb spasticity has been successfully treated with botulinum neurotoxin; however, the injection sites have not been generalized. Sihler’s stain has been used to visualize intramuscular nerve distribution to guide botulinum neurotoxin injection. Sihler staining is a whole-mount nerve staining technique that allows visualization of nerve distribution and mapping of entire nerve supply patterns in skeletal muscle with hematoxylin-stained myelinated nerve fibers. This study reviewed and summarized previous lower extremity spasticity studies to determine the ideal injection site for botulinum neurotoxin.
Literatur
1.
Zurück zum Zitat Arnold AS, Thelen DG, Schwartz MH, Anderson FC, Delp SL (2007) Muscular coordination of knee motion during the terminal-swing phase of normal gait. J Biomech 40(15):3314–3324PubMedPubMedCentralCrossRef Arnold AS, Thelen DG, Schwartz MH, Anderson FC, Delp SL (2007) Muscular coordination of knee motion during the terminal-swing phase of normal gait. J Biomech 40(15):3314–3324PubMedPubMedCentralCrossRef
2.
Zurück zum Zitat Barnes MP, Kent RM, Semlyen JK, McMullen KM (2003) Spasticity in multiple sclerosis. Neurorehabil Neural Repair 17(1):66–70PubMedCrossRef Barnes MP, Kent RM, Semlyen JK, McMullen KM (2003) Spasticity in multiple sclerosis. Neurorehabil Neural Repair 17(1):66–70PubMedCrossRef
3.
Zurück zum Zitat Corry IS, Cosgrove AP, Duffy CM, Taylor TC, Graham HK (1999) Botulinum toxin A in hamstring spasticity. Gait Posture 10(3):206–210PubMedCrossRef Corry IS, Cosgrove AP, Duffy CM, Taylor TC, Graham HK (1999) Botulinum toxin A in hamstring spasticity. Gait Posture 10(3):206–210PubMedCrossRef
4.
Zurück zum Zitat Fietzek UM, Kossmehl P, Schelosky L, Ebersbach G, Wissel J (2014) Early botulinum toxin treatment for spastic pes equinovarus—a randomized double-blind placebo-controlled study. Eur J Neurol 21(8):1089–1095PubMedCrossRef Fietzek UM, Kossmehl P, Schelosky L, Ebersbach G, Wissel J (2014) Early botulinum toxin treatment for spastic pes equinovarus—a randomized double-blind placebo-controlled study. Eur J Neurol 21(8):1089–1095PubMedCrossRef
5.
Zurück zum Zitat Gracies JM, Lugassy M, Weisz DJ, Vecchio M, Flanagan S, Simpson DM (2009) Botulinum toxin dilution and endplate targeting in spasticity: a double-blind controlled study. Arch Phys Med Rehabil 90(1):9-16 e12PubMedCrossRef Gracies JM, Lugassy M, Weisz DJ, Vecchio M, Flanagan S, Simpson DM (2009) Botulinum toxin dilution and endplate targeting in spasticity: a double-blind controlled study. Arch Phys Med Rehabil 90(1):9-16 e12PubMedCrossRef
6.
Zurück zum Zitat Hsu TS, Dover JS, Arndt KA (2004) Effect of volume and concentration on the diffusion of botulinum exotoxin A. Arch Dermatol 140(11):1351–1354PubMedCrossRef Hsu TS, Dover JS, Arndt KA (2004) Effect of volume and concentration on the diffusion of botulinum exotoxin A. Arch Dermatol 140(11):1351–1354PubMedCrossRef
7.
Zurück zum Zitat Johnson CA, Burridge JH, Strike PW, Wood DE, Swain ID (2004) The effect of combined use of botulinum toxin type A and functional electric stimulation in the treatment of spastic drop foot after stroke: a preliminary investigation. Arch Phys Med Rehabil 85(6):902–909PubMedCrossRef Johnson CA, Burridge JH, Strike PW, Wood DE, Swain ID (2004) The effect of combined use of botulinum toxin type A and functional electric stimulation in the treatment of spastic drop foot after stroke: a preliminary investigation. Arch Phys Med Rehabil 85(6):902–909PubMedCrossRef
8.
Zurück zum Zitat Kinnett D (2004) Botulinum toxin A injections in children: technique and dosing issues. Am J Phys Med Rehabil 83(10 Suppl):S59-64PubMedCrossRef Kinnett D (2004) Botulinum toxin A injections in children: technique and dosing issues. Am J Phys Med Rehabil 83(10 Suppl):S59-64PubMedCrossRef
9.
Zurück zum Zitat Kwon JY, Kim JS (2009) Selective blocking of the anterior branch of the obturator nerve in children with cerebral palsy. Am J Phys Med Rehabil 88(1):7–13PubMedCrossRef Kwon JY, Kim JS (2009) Selective blocking of the anterior branch of the obturator nerve in children with cerebral palsy. Am J Phys Med Rehabil 88(1):7–13PubMedCrossRef
11.
Zurück zum Zitat Lee HJ, Lee JH, Yi KH, Kim HJ (2022) Intramuscular innervation of the supraspinatus muscle assessed using Sihler’s staining: potential application in myofascial pain syndrome. Toxins (Basel) 14(5):310PubMedCrossRef Lee HJ, Lee JH, Yi KH, Kim HJ (2022) Intramuscular innervation of the supraspinatus muscle assessed using Sihler’s staining: potential application in myofascial pain syndrome. Toxins (Basel) 14(5):310PubMedCrossRef
12.
Zurück zum Zitat Lee JH, Lee BN, An X, Chung RH, Han SH (2011) Location of the motor entry point and intramuscular motor point of the tibialis posterior muscle: for effective motor point block. Clin Anat 24(1):91–96PubMedCrossRef Lee JH, Lee BN, An X, Chung RH, Han SH (2011) Location of the motor entry point and intramuscular motor point of the tibialis posterior muscle: for effective motor point block. Clin Anat 24(1):91–96PubMedCrossRef
13.
Zurück zum Zitat Lepage D, Parratte B, Tatu L, Vuiller F, Monnier G (2005) Extra- and intramuscular nerve supply of the muscles of the anterior antebrachial compartment: applications for selective neurotomy and for botulinum toxin injection. Surg Radiol Anat 27(5):420–430PubMedCrossRef Lepage D, Parratte B, Tatu L, Vuiller F, Monnier G (2005) Extra- and intramuscular nerve supply of the muscles of the anterior antebrachial compartment: applications for selective neurotomy and for botulinum toxin injection. Surg Radiol Anat 27(5):420–430PubMedCrossRef
14.
Zurück zum Zitat Liem RS, Douwe van Willigen J (1988) In toto staining and preservation of peripheral nervous tissue. Stain Technol 63(2):113–120PubMedCrossRef Liem RS, Douwe van Willigen J (1988) In toto staining and preservation of peripheral nervous tissue. Stain Technol 63(2):113–120PubMedCrossRef
15.
Zurück zum Zitat Oddy MJ, Brown C, Mistry R, Eastwood DM (2006) Botulinum toxin injection site localization for the tibialis posterior muscle. J Pediatr Orthop B 15(6):414–417PubMedCrossRef Oddy MJ, Brown C, Mistry R, Eastwood DM (2006) Botulinum toxin injection site localization for the tibialis posterior muscle. J Pediatr Orthop B 15(6):414–417PubMedCrossRef
16.
Zurück zum Zitat Park ES, Kim HW, Park CI, Rha DW, Park CW (2006) Dynamic foot pressure measurements for assessing foot deformity in persons with spastic cerebral palsy. Arch Phys Med Rehabil 87(5):703–709PubMedCrossRef Park ES, Kim HW, Park CI, Rha DW, Park CW (2006) Dynamic foot pressure measurements for assessing foot deformity in persons with spastic cerebral palsy. Arch Phys Med Rehabil 87(5):703–709PubMedCrossRef
17.
Zurück zum Zitat Rha DW, Cahill-Rowley K, Young J, Torburn L, Stephenson K, Rose J (2016) Biomechanical and clinical correlates of stance-phase knee flexion in persons with spastic cerebral palsy. PM R 8(1):11–18PubMedCrossRef Rha DW, Cahill-Rowley K, Young J, Torburn L, Stephenson K, Rose J (2016) Biomechanical and clinical correlates of stance-phase knee flexion in persons with spastic cerebral palsy. PM R 8(1):11–18PubMedCrossRef
18.
Zurück zum Zitat Rha DW, Yi KH, Park ES, Park C, Kim HJ (2016) Intramuscular nerve distribution of the hamstring muscles: application to treating spasticity. Clin Anat 29(6):746–751PubMedCrossRef Rha DW, Yi KH, Park ES, Park C, Kim HJ (2016) Intramuscular nerve distribution of the hamstring muscles: application to treating spasticity. Clin Anat 29(6):746–751PubMedCrossRef
19.
Zurück zum Zitat Rosales RL, Kong KH, Goh KJ, Kumthornthip W, Mok VC, Delgado-De Los Santos MM, Chua KS, Abdullah SJ, Zakine B, Maisonobe P, Magis A, Wong KS (2012) Botulinum toxin injection for hypertonicity of the upper extremity within 12 weeks after stroke: a randomized controlled trial. Neurorehabil Neural Repair 26(7):812–821PubMedCrossRef Rosales RL, Kong KH, Goh KJ, Kumthornthip W, Mok VC, Delgado-De Los Santos MM, Chua KS, Abdullah SJ, Zakine B, Maisonobe P, Magis A, Wong KS (2012) Botulinum toxin injection for hypertonicity of the upper extremity within 12 weeks after stroke: a randomized controlled trial. Neurorehabil Neural Repair 26(7):812–821PubMedCrossRef
20.
Zurück zum Zitat Sheverdin VA, Hur MS, Won SY, Song WC, Hu KS, Koh KS, Kim HJ (2009) Extra- and intramuscular nerves distributions of the triceps surae muscle as a basis for muscle resection and botulinum toxin injections. Surg Radiol Anat 31(8):615–621PubMedCrossRef Sheverdin VA, Hur MS, Won SY, Song WC, Hu KS, Koh KS, Kim HJ (2009) Extra- and intramuscular nerves distributions of the triceps surae muscle as a basis for muscle resection and botulinum toxin injections. Surg Radiol Anat 31(8):615–621PubMedCrossRef
21.
Zurück zum Zitat Sihler C (1895) Ueber Muskelspindeln und intramuskuläre Nervenendigungen bei Schlangen und Fröschen. Arch Mikrosk Anat 46(1):709–723CrossRef Sihler C (1895) Ueber Muskelspindeln und intramuskuläre Nervenendigungen bei Schlangen und Fröschen. Arch Mikrosk Anat 46(1):709–723CrossRef
22.
Zurück zum Zitat Van Campenhout A, Verhaegen A, Pans S, Molenaers G (2013) Botulinum toxin type A injections in the psoas muscle of children with cerebral palsy: muscle atrophy after motor end plate-targeted injections. Res Dev Disabil 34(3):1052–1058PubMedCrossRef Van Campenhout A, Verhaegen A, Pans S, Molenaers G (2013) Botulinum toxin type A injections in the psoas muscle of children with cerebral palsy: muscle atrophy after motor end plate-targeted injections. Res Dev Disabil 34(3):1052–1058PubMedCrossRef
23.
Zurück zum Zitat Williams TW Jr (1943) A technique for the gross differential staining of peripheral nerves in cleared vertebrate tissue. Anat Rec 86(2):189–195CrossRef Williams TW Jr (1943) A technique for the gross differential staining of peripheral nerves in cleared vertebrate tissue. Anat Rec 86(2):189–195CrossRef
24.
Zurück zum Zitat Won SY, Kim DH, Yang HM, Park JT, Kwak HH, Hu KS, Kim HJ (2011) Clinical and anatomical approach using Sihler’s staining technique (whole mount nerve stain). Anat Cell Biol 44(1):1–7PubMedPubMedCentralCrossRef Won SY, Kim DH, Yang HM, Park JT, Kwak HH, Hu KS, Kim HJ (2011) Clinical and anatomical approach using Sihler’s staining technique (whole mount nerve stain). Anat Cell Biol 44(1):1–7PubMedPubMedCentralCrossRef
25.
Zurück zum Zitat Won SY, Rha DW, Kim HS, Jung SH, Park ES, Hu KS, Kim HJ (2012) Intramuscular nerve distribution pattern of the adductor longus and gracilis muscles demonstrated with Sihler staining: guidance for botulinum toxin injection. Muscle Nerve 46(1):80–85PubMedCrossRef Won SY, Rha DW, Kim HS, Jung SH, Park ES, Hu KS, Kim HJ (2012) Intramuscular nerve distribution pattern of the adductor longus and gracilis muscles demonstrated with Sihler staining: guidance for botulinum toxin injection. Muscle Nerve 46(1):80–85PubMedCrossRef
26.
Zurück zum Zitat Yi K-H, Cong L, Bae J-H, Park ES, Rha D-W, Kim H-J (2017) Neuromuscular structure of the tibialis anterior muscle for functional electrical stimulation. Surg Radiol Anat 39(1):77–83PubMedCrossRef Yi K-H, Cong L, Bae J-H, Park ES, Rha D-W, Kim H-J (2017) Neuromuscular structure of the tibialis anterior muscle for functional electrical stimulation. Surg Radiol Anat 39(1):77–83PubMedCrossRef
27.
Zurück zum Zitat Yi K-H, Lee H-J, Choi Y-J, Lee J-H, Hu K (2020) Intramuscular neural distribution of rhomboid muscles: evaluation for botulinum toxin injection using modified Sihler’s method. Toxins 12:289PubMedPubMedCentralCrossRef Yi K-H, Lee H-J, Choi Y-J, Lee J-H, Hu K (2020) Intramuscular neural distribution of rhomboid muscles: evaluation for botulinum toxin injection using modified Sihler’s method. Toxins 12:289PubMedPubMedCentralCrossRef
28.
Zurück zum Zitat Yi K-H, Lee H-J, Hur H-W, Seo K, Kim H-J (2022) Guidelines for botulinum neurotoxin injection for facial contouring. Plast Reconstr Surg 150(3):562e–571ePubMedCrossRef Yi K-H, Lee H-J, Hur H-W, Seo K, Kim H-J (2022) Guidelines for botulinum neurotoxin injection for facial contouring. Plast Reconstr Surg 150(3):562e–571ePubMedCrossRef
29.
Zurück zum Zitat Yi K-H, Lee H-J, Seo KK, Kim H-J (2022) Intramuscular neural arborization of the latissimus dorsi muscle: application of botulinum neurotoxin injection in flap reconstruction. Toxins 14(2):107PubMedPubMedCentralCrossRef Yi K-H, Lee H-J, Seo KK, Kim H-J (2022) Intramuscular neural arborization of the latissimus dorsi muscle: application of botulinum neurotoxin injection in flap reconstruction. Toxins 14(2):107PubMedPubMedCentralCrossRef
30.
Zurück zum Zitat Yi K-H, Lee J-H, Hu H-W, Kim H-J (2022) Novel anatomical guidelines on botulinum neurotoxin injection for wrinkles in the nose region. Toxins 14(5):342PubMedPubMedCentralCrossRef Yi K-H, Lee J-H, Hu H-W, Kim H-J (2022) Novel anatomical guidelines on botulinum neurotoxin injection for wrinkles in the nose region. Toxins 14(5):342PubMedPubMedCentralCrossRef
31.
Zurück zum Zitat Yi K-H, Lee J-H, Kim G-Y, Yoon S-W, Oh W, Kim H-J (2022) Novel anatomical proposal for botulinum neurotoxin injection targeting lateral canthal rhytids. Toxins 14(7):462PubMedPubMedCentralCrossRef Yi K-H, Lee J-H, Kim G-Y, Yoon S-W, Oh W, Kim H-J (2022) Novel anatomical proposal for botulinum neurotoxin injection targeting lateral canthal rhytids. Toxins 14(7):462PubMedPubMedCentralCrossRef
32.
Zurück zum Zitat Yi KH, Choi YJ, Cong L, Lee K-L, Hu KS (2019) Effective botulinum toxin injection guide for treatment of cervical dystonia. Clin Anat 33(2):192–198PubMedCrossRef Yi KH, Choi YJ, Cong L, Lee K-L, Hu KS (2019) Effective botulinum toxin injection guide for treatment of cervical dystonia. Clin Anat 33(2):192–198PubMedCrossRef
33.
Zurück zum Zitat Yi KH, Lee HJ, Choi YJ, Lee K, Lee JH, Kim HJ (2021) Anatomical guide for botulinum neurotoxin injection: application to cosmetic shoulder contouring, pain syndromes, and cervical dystonia. Clin Anat 34(6):822–828PubMedCrossRef Yi KH, Lee HJ, Choi YJ, Lee K, Lee JH, Kim HJ (2021) Anatomical guide for botulinum neurotoxin injection: application to cosmetic shoulder contouring, pain syndromes, and cervical dystonia. Clin Anat 34(6):822–828PubMedCrossRef
34.
Zurück zum Zitat Yi KH, Lee HJ, Lee JH, Lee KL, Kim HJ (2021) Effective botulinum neurotoxin injection in treating iliopsoas spasticity. Clin Anat 34(3):431–436PubMedCrossRef Yi KH, Lee HJ, Lee JH, Lee KL, Kim HJ (2021) Effective botulinum neurotoxin injection in treating iliopsoas spasticity. Clin Anat 34(3):431–436PubMedCrossRef
35.
Zurück zum Zitat Yi KH, Lee HJ, Lee JH, Seo KK, Kim HJ (2021) Application of botulinum neurotoxin injections in TRAM flap for breast reconstruction: intramuscular neural arborization of the rectus abdominis muscle. Toxins 13(4):269PubMedPubMedCentralCrossRef Yi KH, Lee HJ, Lee JH, Seo KK, Kim HJ (2021) Application of botulinum neurotoxin injections in TRAM flap for breast reconstruction: intramuscular neural arborization of the rectus abdominis muscle. Toxins 13(4):269PubMedPubMedCentralCrossRef
36.
Zurück zum Zitat Yi KH, Lee HJ, Seo KK, Kim HJ (2022) Botulinum neurotoxin injection guidelines regarding flap surgeries in breast reconstruction. J Plast Reconstr Aesthet Surg 75(1):503–505PubMedCrossRef Yi KH, Lee HJ, Seo KK, Kim HJ (2022) Botulinum neurotoxin injection guidelines regarding flap surgeries in breast reconstruction. J Plast Reconstr Aesthet Surg 75(1):503–505PubMedCrossRef
37.
38.
Zurück zum Zitat Yi KH, Lee JH, Kim HJ (2022) Intramuscular neural distribution of the serratus anterior muscle: regarding botulinum neurotoxin injection for treating myofascial pain syndrome. Toxins 14(4):271PubMedPubMedCentralCrossRef Yi KH, Lee JH, Kim HJ (2022) Intramuscular neural distribution of the serratus anterior muscle: regarding botulinum neurotoxin injection for treating myofascial pain syndrome. Toxins 14(4):271PubMedPubMedCentralCrossRef
39.
Zurück zum Zitat Yi KH, Lee JH, Kim HM, Kim HJ (2022) The botulinum neurotoxin for pain control after breast reconstruction: neural distribution of the pectoralis major muscle. Reg Anesth Pain Med 47(5):322–326PubMedCrossRef Yi KH, Lee JH, Kim HM, Kim HJ (2022) The botulinum neurotoxin for pain control after breast reconstruction: neural distribution of the pectoralis major muscle. Reg Anesth Pain Med 47(5):322–326PubMedCrossRef
40.
Zurück zum Zitat Yi KH, Lee JH, Lee DK, Hu HW, Seo KK, Kim HJ (2021) Anatomical locations of the motor endplates of sartorius muscle for botulinum toxin injections in treatment of muscle spasticity. Surg Radiol Anat 43(12):2025–2030PubMedPubMedCentralCrossRef Yi KH, Lee JH, Lee DK, Hu HW, Seo KK, Kim HJ (2021) Anatomical locations of the motor endplates of sartorius muscle for botulinum toxin injections in treatment of muscle spasticity. Surg Radiol Anat 43(12):2025–2030PubMedPubMedCentralCrossRef
41.
Zurück zum Zitat Yi KH, Lee JJ, Hur HW, Bae HK, Kim H-J (2022) Hyaluronic acid filler injection for deep nasolabial folds: a novel intraoral approach. Clin Anat 35(6):820–823PubMedCrossRef Yi KH, Lee JJ, Hur HW, Bae HK, Kim H-J (2022) Hyaluronic acid filler injection for deep nasolabial folds: a novel intraoral approach. Clin Anat 35(6):820–823PubMedCrossRef
42.
Zurück zum Zitat Yi KH, Lee KL, Lee JH, Hu HW, Kim HJ (2022) Guidance to trigger point injection for treating myofascial pain syndrome: Intramuscular neural distribution of the quadratus lumborum. Clin Anat 35(8):1100–1106PubMedCrossRef Yi KH, Lee KL, Lee JH, Hu HW, Kim HJ (2022) Guidance to trigger point injection for treating myofascial pain syndrome: Intramuscular neural distribution of the quadratus lumborum. Clin Anat 35(8):1100–1106PubMedCrossRef
43.
Zurück zum Zitat Yi KH, Lee KL, Lee JH, Hu HW, Lee K, Seo KK, Kim HJ (2021) Guidelines for botulinum neurotoxin injections in piriformis syndrome. Clin Anat 34(7):1028–1034PubMedCrossRef Yi KH, Lee KL, Lee JH, Hu HW, Lee K, Seo KK, Kim HJ (2021) Guidelines for botulinum neurotoxin injections in piriformis syndrome. Clin Anat 34(7):1028–1034PubMedCrossRef
44.
Zurück zum Zitat Yi KH, Rha DW, Lee SC, Cong L, Lee HJ, Lee YW, Kim HJ, Hu KS (2016) Intramuscular nerve distribution pattern of ankle invertor muscles in human cadaver using sihler stain. Muscle Nerve 53(5):742–747PubMedCrossRef Yi KH, Rha DW, Lee SC, Cong L, Lee HJ, Lee YW, Kim HJ, Hu KS (2016) Intramuscular nerve distribution pattern of ankle invertor muscles in human cadaver using sihler stain. Muscle Nerve 53(5):742–747PubMedCrossRef
Metadaten
Titel
Guidance in botulinum neurotoxin injection for lower extremity spasticity: Sihler’s staining technique
verfasst von
Hyewon Hu
Min Ho An
Hyung-Jin Lee
Kyu-Ho Yi
Publikationsdatum
09.06.2023
Verlag
Springer Paris
Erschienen in
Surgical and Radiologic Anatomy / Ausgabe 8/2023
Print ISSN: 0930-1038
Elektronische ISSN: 1279-8517
DOI
https://doi.org/10.1007/s00276-023-03178-9

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