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30.04.2022 | Original Article

Effect of Acid or Laser Treatment on Degradation of Dentin Matrix

verfasst von: Aslihan Usumez, Tugrul Sari, Roda Seseogullari Dirihan, Mehmet Esad Guven, Serra Oguz Ahmet, Norbert Gutknecht, Arzu Tezvergil Mutluay

Erschienen in: Lasers in Dental Science | Ausgabe 2/2022

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Abstract

Purpose

The objective of this study was to determine the effect of dental laser (DL) irradiation on the metalloproteinases (MMP)-mediated dentin collagen degradation.

Methods

Human dentin disks were prepared (0.3 × 5x6mm, n = 60) and subjected to different treatments (n = 10/group): (1) control group (no treatment); (2) application of 37% phosphoric acid (PA) for 15 s; (3) irradiation with Er:YAG laser (120 mJ, 10 Hz); (4) irradiation with Er:YAG laser (120 mJ, 20 Hz); (5) irradiation with Er,Cr:YSGG laser (130mj, 30 Hz); and (6) irradiation with Er,Cr:YSGG laser (80mj, 50 Hz). After treatment, the total MMP activity and dry mass of each beam were measured, and after rehydration, they were incubated in 1 mL calcium- and zinc-containing incubation media for (a) one week and (b) three weeks. After each time point, dry mass changes were reassessed, and the aliquots of the incubation media were analyzed for pyridinoline-cross-link-containing degradation fragment of the C-terminal telopeptide of type I collagen (ICTP) using ELISA kits. All data were compared using ANOVA and Tukey’s test.

Results

ANOVA showed significant differences among tested groups (p < 0.001). Laser-treated beams showed 55–75% less activity compared to 37% phosphoric acid-treated beams. The highest ICTP values were seen when PA was applied to the dentin surface in both time periods (p < 0.05). DL irradiation, be it with Er:YAG or Er,Cr:YSGG laser, did not increase the ICTP values in both time periods when compared with the control group (p > 0.05).

Conclusion

The MMP-mediated dentin collagen degradation was the highest when PA was applied to the dentin surface and the lowest in Er:YAG or Er,Cr:YSGG laser-treated dentin groups in both time periods.

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Titel: Effect of Acid or Laser Treatment on Degradation of Dentin Matrix
verfasst von: Aslihan Usumez
Tugrul Sari
Roda Seseogullari Dirihan
Mehmet Esad Guven
Serra Oguz Ahmet
Norbert Gutknecht
Arzu Tezvergil Mutluay
Publikationsdatum: 30.04.2022
Verlag: Springer International Publishing
Erschienen in: Lasers in Dental Science / Ausgabe 2/2022
Elektronische ISSN: 2367-2587
DOI: https://doi.org/10.1007/s41547-022-00155-0

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Effect of Acid or Laser Treatment on Degradation of Dentin Matrix