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01.06.2024 | Research

Assessment of multispecies biofilm growth on root canal dentin under different radiation therapy regimens

verfasst von: Taynara Santos Goulart, Tainara Hawerroth, Cleonice da Silveira Teixeira, Karina Cesca, Rodrigo Rohenkohl Silva, Rafael Ratto de Moraes, Mariana Comparotto Minamisako, Marcio Toshio Umeda Takashima, Nayara Cardoso Cábia, Eduardo Antunes Bortoluzzi, Ricardo Ruiz Mazzon, Josiane de Almeida, Lucas da Fonseca Roberti Garcia

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2024

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Abstract

Objectives

To assess the growth of a multispecies biofilm on root canal dentin under different radiotherapy regimens.

Materials and methods

Sixty-three human root dentin cylinders were distributed into six groups. In three groups, no biofilm was formed (n = 3): NoRT) non-irradiated dentin; RT55) 55 Gy; and RT70) 70 Gy. In the other three groups (n = 18), a 21-day multispecies biofilm (Enterococcus faecalis, Streptococcus mutans, and Candida albicans) was formed in the canal: NoRT + Bio) non-irradiated + biofilm; RT55 + Bio) 55 Gy + biofilm; and RT70 + Bio) 70 Gy + biofilm. The biofilm was quantified (CFUs/mL). Biofilm microstructure was assessed under SEM. Microbial penetration into dentinal tubules was assessed under CLSM. For the biofilm biomass and dentin microhardness pre- and after biofilm growth assessments, 45 bovine dentin specimens were distributed into three groups (n = 15): NoRT) non-irradiated + biofilm; RT55 + Bio) 55 Gy + biofilm; and RT70 + Bio) 70 Gy + biofilm.

Results

Irradiated specimens (70 Gy) had higher quantity of microorganisms than non-irradiated (p = .010). There was gradual increase in biofilm biomass from non-irradiated to 55 Gy and 70 Gy (p < .001). Irradiated specimens had greater reduction in microhardness after biofilm growth. Irradiated dentin led to the growth of a more complex and irregular biofilm. There was microbial penetration into the dentinal tubules, regardless of the radiation regimen.

Conclusion

Radiotherapy increased the number of microorganisms and biofilm biomass and reduced dentin microhardness. Microbial penetration into dentinal tubules was noticeable.

Clinical relevance

Cumulative and potentially irreversible side effects of radiotherapy affect biofilm growth on root dentin. These changes could compromise the success of endodontic treatment in oncological patients undergoing head and neck radiotherapy.

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Titel: Assessment of multispecies biofilm growth on root canal dentin under different radiation therapy regimens
verfasst von: Taynara Santos Goulart
Tainara Hawerroth
Cleonice da Silveira Teixeira
Karina Cesca
Rodrigo Rohenkohl Silva
Rafael Ratto de Moraes
Mariana Comparotto Minamisako
Marcio Toshio Umeda Takashima
Nayara Cardoso Cábia
Eduardo Antunes Bortoluzzi
Ricardo Ruiz Mazzon
Josiane de Almeida
Lucas da Fonseca Roberti Garcia
Publikationsdatum: 01.06.2024
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2024
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-024-05719-x

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Assessment of multispecies biofilm growth on root canal dentin under different radiation therapy regimens