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18.06.2021 | Original Article

An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

verfasst von: Sarita Giri, Ayuko Takada, Durga Paudel, Koki Yoshida, Masae Furukawa, Yasuhiro Kuramitsu, Kenji Matsushita, Yoshihiro Abiko, Yasushi Furuichi

Erschienen in: Odontology | Ausgabe 1/2022

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Abstract

Gingival tissue shows progressive changes with aging and an in vitro model of gingival tissue could be useful in understanding age-associated oral diseases. The present study aims to establish a hydrogen peroxide (H₂O₂) treatment model to induce aging in human gingival epithelial cells. In addition, fisetin, a flavonoid component studied for the anti-aging property is used to examine if it could reverse the induced senescence. Primary human gingival epithelial progenitor (HGEPp) cells were cultured and treated with different concentrations of H₂O₂. A cell vitality and morphology, senescence-associated beta-galactosidase (SA-β-gal) staining, mRNA and protein expression analysis of known senescence markers p16, p21, and p53, and cell cycle assay were performed. The cells showed dose-dependent changes in vitality and morphology, SA-β-gal staining, relative mRNA and protein expression, and cell cycle assay after H₂O₂ treatment. Based on these results, 400 μM H₂O₂ was considered as an optimal concentration to induce senescence. Treatment of senescence-induced cells with fisetin downregulated all the senescence markers used in this study. In conclusion, a senescence model of gingival epithelial cells induced by hydrogen peroxide treatment was established which could be employed to study age-related periodontal diseases.

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Titel: An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment
verfasst von: Sarita Giri
Ayuko Takada
Durga Paudel
Koki Yoshida
Masae Furukawa
Yasuhiro Kuramitsu
Kenji Matsushita
Yoshihiro Abiko
Yasushi Furuichi
Publikationsdatum: 18.06.2021
Verlag: Springer Singapore
Erschienen in: Odontology / Ausgabe 1/2022
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-021-00630-3

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An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

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